Screening of Organophosphorus Acid Anhydrases from different Sources by Western Blot Analysis
Abstract
Enzymes that are capable of degrading organophosphorous compounds (e. g., acetylcholinesterase inhibitors, pesticides, and G-type agents) have been found in both procaryotic and eukaryotic organisms. These enzymes, referred to as organophosphorous acid anhydrases (OPA anhydrases), offer considerable potential for use in chemical agent decontamination systems. One of these enzymes, OPAA-2 from halophilic JD6.5, has recently been purified to homogeneity. The polyclonal and monoclonal antibodies against OPAA-2 have been prepared and shown to react specifically with this enzyme. Using this monoclonal antibody as a probe to screen OPA anhydrases from various sources in Western blot analysis, it was found that microorganisms with high levels of OPA anhydrase can be recognized by their unique binding ability.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1991
- Accession Number
- ADA233635
Entities
People
- J. Defrank
- Madeleine L. Miller
- T. C. Cheng
Organizations
- Edgewood Chemical Biological Center