'Coxiella Burnetii' Vaccine Development: Lipopolysaccharide Structural Analysis
Abstract
Analytical instrumentation and methodology is presented for the determination of endotoxin-related structures at much improved sensitivity and specificity using techniques in chromatography and mass spectrometry. These techniques include a newly synthesized reagent that has provided femtomole sensitivity for oligosaccharide detection; and a preanalysis chemical approach for the determination of oligosaccharide linkage, branching, and sequence. These approaches are compatible with supercritical fluid chromatography-mass spectrometry (SFC-MS) and combine to provide the elements of a global approach to oligosaccharide structure. The utility of this approach has been demonstrated for glycolipids and N-linked glycans. The lipid-A structure from Salmonella minnesota has been demonstrated and the report outlines the currently understood structure Coxiella burnetii lipid-A.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 20, 1991
- Accession Number
- ADA233705
Entities
People
- Vernon N. Reinhold
Organizations
- Harvard T.H. Chan School of Public Health