Neurotoxin and Epitope Structural Studies
Abstract
Neurotoxins of botulinum clostridium are scientifically interesting for two reasons. First, they are extremely toxic. Second, they can be used as models for three important biological phenomena, selective recognition by a target cell, transport through the plasma membrane and toxic activity. All three activities are situated on one polypeptide of approx. 150 kDa. Whereas the complete gene sequences of neurotoxins A and C1 were published very recently only parts of the neurotoxin E sequence is known. By using mass spectrometry, supported by automated Edman degradation we were able to deduce approx. 5Q kDa of well established sequence information. Additionally, we also found approx. 30Q kDa of preliminary sequence information.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 25, 1991
- Accession Number
- ADA233710
Entities
People
- Donald F. Hunt
Organizations
- University of Virginia