Interaction of Electromagnetic Fields with Biosynthetic Processes in Connective Tissue Cells

Abstract

The specific objectives of this research period were (1) determine whether chondrocytes can be maintained in long-term (2-3 months) culture in agarose gel in order to test the effects of chronic as well as acute response to exposure to electromagnetic fields; (2) measure chondrocyte proliferation, biosynthetic rates for proteoglycans and proteins, total accumulation of proteoglycans, and physical properties of agarose/chondrocyte gel cultures; (3) construct specialized chambers for application of electric fields to agarose/ chondrocyte disks in a standard incubator environment; and (4) begin experiments to measure the effects of applied current densities over a wide range of amplitudes and frequencies on biosynthesis of proteoglycans and proteins.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1989
Accession Number
ADA234221

Entities

People

  • Alan J. Grodzinsky
  • Michael D. Buschmann
  • Yehezkiel A. Gluzband

Organizations

  • Massachusetts Institute of Technology

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Boltzmann Equation
  • Cartilage
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Connective Tissue
  • Connective Tissue Cells
  • Deoxyribonucleic Acids
  • Electric Fields
  • Electromagnetic Fields
  • Governments
  • Mechanical Properties
  • New York
  • Physical Properties
  • Polysaccharides
  • Standards

Readers

  • Immunology and Pathology
  • Molecular and Cellular Biochemistry
  • Plasma Physics.