Demonstration of the Origin of Human Mast Cells from CD34+ Bone Marrow Progenitor Cells

Abstract

It has been established that murine mast cells are derived from a pluripotent bone marrow stem cell. In humans, the corresponding pluripotent cell is included in the CD34 bone marrow population. To determine whether human mast cells arise from CD34 human progenitor cells, enriched CD34 cells were cultured over agarose surfaces(Interphase cultures) or cocultured with mouse 3T3 fibroblasts in the presence of recombinant human (rh) IL-3. In the mouse, mast cell progenitor cells have been shown to originate in bone marrow and form colonies (CFU-S) in mouse spleen. These mast cell progenitor cells selectively adhere to mouse embryonic skin monolayers, express mRNA for the subunits of IgE receptors before phenotypic differentiation and proliferate in the presence of IL-3 and IL-4. In humans, mast cells and basophils have been cultured from bone marrow in agarose interphase cultures.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1991
Accession Number
ADA234815

Entities

People

  • Arnold S. Kirshenbaum
  • Dean D. Metcalfe
  • Julie P. Goff
  • Steven W. Kessler

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Allergy And Immunology
  • Availability
  • Blood
  • Bone Marrow
  • Bone Marrow Cells
  • Cardiovascular System
  • Cell Count
  • Cells
  • Connective Tissue
  • Culture Media
  • Culture Techniques
  • Cultured Cells
  • Granulocytes
  • Lymphocytes
  • Mast Cells
  • Stem Cells
  • Tissues

Fields of Study

  • Biology

Readers

  • Allergy and Immunology.
  • Molecular Genetics
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech