Possible Mechanism for Denervation Effect on Wound Healing.
Abstract
The iron-transport plasma protein transferrin is required for cell proliferation and is present in peripheral nerves. WE have tested the hypothesis that release of this factor from axons may be involved in the growth-promoting effect neurons exert on cell proliferation during certain processes of repair in poorly vascularized issues. Using regeneration in an amphibian as the model system, transferrin was purified, antibodies against it were produced, and the factor was studied qualitatively and quantitatively during nerve and limb regeneration. Immunocytochemistry showed the protein to be present in axons and Schwann cells, as well as in the perineurium of peripheral nerves. An immunoassay developed in the project revealed that the transferrin content of sciatic nerves increases dramatically during regeneration and that the protein is included in the anterograde fast transport component of axons. These results are consistent with the hypothesis that neural release of this factor may be involved in the trophic effect of nerves observed during regeneration.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 14, 1990
- Accession Number
- ADA235214
Entities
People
- Anthony L. Mescher
Organizations
- Indiana University