Wound Healing by Cultured Skin Cells and Growth Factors.
Abstract
The studies described in this report were aimed at the biochemical characterization and purification of a wound healing growth factor, named Epidermal Cell derived Factor (EDF). The biological activity of EDF was found to embody enhancement of epidermal regeneration and regulation of dermal repair in skin wounds. We have accomplished the following: A tissue culture bioassay that is based on the capability of fibroblasts to contract collagen gels was shown to reflect in vivo effects of EDF on dermal cells and was adopted for examination of large numbers of samples during biochemical purification steps. Supernatant fluids of epidermal cell cultures and cell extracts of epidermal cells grown in tissue culture were tested as possible sources for purification of the factor, but substituted by epidermal cells obtained from animals following a superficial wounding. Cell extracts prepared from such regenerating epidermal cells have been found to be the richest source from all tissues tested.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 28, 1990
- Accession Number
- ADA235300
Entities
People
- Magdalena Eisinger
Organizations
- Memorial Sloan Kettering Cancer Center