18 SrRNA Degradation is Not Accompanied by Altered rRNA Transport at Early Times Following Irradiation of HeLa Cells
Abstract
In recent investigations on the effects of radiation on rRNA processing in HeLa S3 cells, we pulse-labeled the cells with uridine immediately prior to irradiation. The 45 S rRNA precursor, which undergoes nuclear processing to form one each of its major daughter species, 28 S and 18 S rRNA, was separated from the daughter species by gel electrophoresis and the radiolabel in each species determined at various times after irradiation. By pulse-labeling the cells prior to irradiation, superimposed effects caused by radiation-induced alterations of rRNA transcription and Refs. therein were minimized, permitting selective analysis of the processing of that fraction of 45 S precursor that had been synthesized (radiolabeled) predominantly prior to irradiation. We now report more detailed studies on 45 S rRNA processing within the first 2 h following irradiation in which we have found a maximum 28 S:18 S ratio of 2:1 that is observed about 1 h following irradiation of 5 or 10 Gy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1990
- Accession Number
- ADA235466
Entities
People
- David Mcclain
- John M. Krolak
- Kenneth W. Minton
- Pinhas Fuchs
Organizations
- Armed Forces Radiobiology Research Institute