Free Radicals Mediate Peroxidative Damage in Guinea Pig Hippocampus in vitro

Abstract

Previous studies have shown that peroxide caused electrophysiological damage. The present study investigates the action of agents that interfere with a free radical process in an effort to define the mechanism of peroxide damage. Deferoxamine chelates iron, making it unavailable for the Fenton reaction and thereby preventing the formation of hydroxyl free radicals from peroxide. Dimethylsulfoxide (DMSO) scavenges hydroxyl free radicals. Trolox-C, a water soluble Vitamin E analog, is an antioxidant that can scavenge peroxy radicals. Slices of hippcampus were removed from brains of euthanized guinea pigs. Electrical stimulation of an orthodromic pathway to CA1 region evoked a synaptic response and a population spike. Input-output curves were generated to evaluate the protection by deferoxamine, Trolox C, and DMSO on the synaptic damage and impaired spike generation caused by peroxide. Lipid peroxidation was measured by the thiobarbituric acid test.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1989
Accession Number
ADA235472

Entities

People

  • K. H. Lee
  • K. L. Neel
  • T. C. Pellmar

Organizations

  • Armed Forces Radiobiology Research Institute

Tags

Communities of Interest

  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Amplitude
  • Analogs
  • Animals
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Free Radicals
  • Hydroxyl Radical
  • Membrane Lipids
  • Metals
  • Oxides
  • Radiation
  • Rocket Oxidizers
  • Rodents
  • Thiobarbituric Acid
  • Transition Metals
  • Vitamin E

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Neuroscience
  • Organic Chemistry