Effect of Adherence, Cell Morphology, and Lipopolysaccharide on Potassium Conductance and Passive Membrane Properties of Murine Macrophage J774. 1 Cells
Abstract
The effect of adherence, cell morphology, and lipopolysaccharide on electrical membrane properties and on the expression of the inwardly rectifying K conductance in J774.1 cells were investigated. Whole-cell inwardly rectifying K current membrane capacitance and membrane potential were measured using the patch-clamp technique. Specific conductance was measured as a function of time after adherence, and was found to increase almost twofold one day after plating. Membrane properties were analyzed as a function of cell morphology, by dividing cells into three categories ranging from small round cells to large, extremely spread cells. The capacitance of spread cells increased more than twofold within one day after adherence, which indicates that spread cells inserted new membrane.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1990
- Accession Number
- ADA235474
Entities
People
- Elaine K. Gallin
- Leslie C. Mckinney
Organizations
- Armed Forces Radiobiology Research Institute