Light-Addressable Potentiometric (LAP) Sensor Assay of Newcastle Disease Virus

Abstract

A sandwich immunoassay for Newcastle Disease Virus (NDV) was developed using streptavidin-biotin mediated filtration capture and silicon- based semiconductor detection. The assays were carried out in one step whereby the immunoreagents and the antigen (NDV) were mixed together and incubated. The pH sensing capability of the detector, a light addressable potentiometric (LAP) sensor, was employed to detect the presence of immobilized urease-conjugated antibodies. Lower limits of detection (LOD) of the assay were determined as a function of incubation time. The LOD were approximately 27, 15 and 3 ng per well for 1, 5 and 60 minute incubations, respectively. The techniques described here have the potential to be adapted to an automated LAP sensor for use in the BioChemical Detector program.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1991
Accession Number
ADA236433

Entities

People

  • Jonathan P. Wong
  • R. E. Fulton
  • William E. Lee

Organizations

  • Defence Research and Development Canada

Tags

Communities of Interest

  • Sensors

DTIC Thesaurus Topics

  • Abstracts
  • Albumins
  • Animal Diseases
  • Antigen-Antibody Complex
  • Antigens
  • Biological Detection
  • Calibration
  • Coefficients
  • Contracts
  • Detection
  • Detectors
  • Diseases And Disorders
  • Models
  • Proteins
  • Regression Analysis
  • Sensitivity
  • Standards

Readers

  • Molecular and Cellular Biochemistry
  • Sensor Fusion and Tracking Systems.
  • Virology (or Medical Virology).

Technology Areas

  • Microelectronics
  • Microelectronics - Microelectromechanical Systems