Immunoassay of Mouse Immunoglobulin G by a Light-Addressable Potentiometric Sensor

Abstract

A sensitive enzyme immunoassay for the quantitation of mouse immunoglobulin G (mIgG) was developed using a light-addressable potentiometric (LAP) sensor as the detection system. The assay was carried out on nitrocellulose membrane filters and used sandwich-format one-step incubations of antigens and antibodies. In addition, the assay employed a high affinity biotin- streptavidin interaction to capture the immunocomplexes onto nitrocellulose membranes, and a urease-conjugated anti-mouse IgF detector antibody.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1991
Accession Number
ADA236434

Entities

People

  • H. G. Thompson
  • William E. Lee

Organizations

  • Defence Research and Development Canada

Tags

Communities of Interest

  • Sensors

DTIC Thesaurus Topics

  • Abstracts
  • Accuracy
  • Albumins
  • Antibodies
  • Calibration
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Detectors
  • Engineering
  • Immunoglobulins
  • Materials
  • Measurement
  • Molecular Weight
  • Reproducibility
  • Standards
  • United States

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry