Immunoassay of Mouse Immunoglobulin G by a Light-Addressable Potentiometric Sensor
Abstract
A sensitive enzyme immunoassay for the quantitation of mouse immunoglobulin G (mIgG) was developed using a light-addressable potentiometric (LAP) sensor as the detection system. The assay was carried out on nitrocellulose membrane filters and used sandwich-format one-step incubations of antigens and antibodies. In addition, the assay employed a high affinity biotin- streptavidin interaction to capture the immunocomplexes onto nitrocellulose membranes, and a urease-conjugated anti-mouse IgF detector antibody. The lower limits of detection of mIgG by this assay were 300, 100, and 10 pg per well, respectively, for 1, 5, and 60 min incubations. The time required for the assay was the incubation time plus 4-5 min, in total for the mixing, washing and detection procedures.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1991
- Accession Number
- ADA236613
Entities
People
- H. G. Thompson
- William E. Lee
Organizations
- Defence Research and Development Canada