Long-Term Risk Function Evaluation: Biochemical
Abstract
A method of determining the true level of HDL-cholesterol in serum was developed. The serum lipoproteins were titrated by adding increasing amounts of lipoprotein precipitant, measuring the cholesterol content in the supernate, and monitoring the lipoprotein content of the supernate by agarose gel electrophoresis. For quality control of routine HDL-cholesterol measurements, serum pools proved highly useful. A computer program that printed out values obtained for total cholesterol, HDL-cholesterol, and triglyceride levels during the last 10 laboratory runs, plus statistical information on the levels and the ratios between those levels, was a useful tool in identifying sources of error and was a strong motivating factor in technician morale. Various methods were used separately and in combination in unsuccessful efforts to develop a routine procedure for measuring the composition of serum lipoproteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1990
- Accession Number
- ADA236945
Entities
People
- Dale A. Clark
Organizations
- United States Air Force School of Aerospace Medicine