Debaryomyces hansenii: A Model System for Marine Molecular Biology

Abstract

In an attempt to construct plasmids with the capability of autonomous replication we have isolated two ARS from Debaryomyces utilizing S. cerevisiae strain SEY 2108 (Ura3) and its corresponding gene URA3 as a marker. Attempts are now being made to transform Ura3 Debaryomyces with this plasmid. Transformation attempted with techniques such as protoplasting, alkali cations and electroporation has not met with success. With the arrival of strain J-26 for Dr. L. Adler, transformation will be attempted utilizing hygromycin resistance as a marker. Dr. Adlers has also agreed to generate Ura3 mutants of his strain for use as a biological marker. To generate auxotrophic mutations for transformation, mutagenesis was performed utilizing Ethyl Methyl Sulfonate.

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Document Details

Document Type
Technical Report
Publication Date
May 30, 1991
Accession Number
ADA236966

Entities

People

  • Govind S. Nadathur

Organizations

  • University of California, Santa Barbara

Tags

Communities of Interest

  • Autonomy

DTIC Thesaurus Topics

  • Biological Markers
  • Biology
  • Chain Reactions
  • Chemical Compounds
  • Chemical Reactions
  • Chemistry
  • Genes
  • Genetics
  • Heterocyclic Acids
  • Molecular Biology
  • Polymerase Chain Reaction
  • Recombinant Dna
  • Sequence Analysis
  • Sequences
  • Students
  • Universities

Readers

  • Electronics Engineering
  • Molecular Genetics
  • Organic Chemistry