Further Development and Validation of the frog Embryo Teratogenesis Assay - Xenopus (FETAX)

Abstract

The goal of the project was to develop and validate the Frog Embryo Teratogenesis Assay- Xenopus (FETAX). FETAX is a 96 h whole embryo bioassay designed to rapidly assess the developmental toxicity of pure compounds and complex mixtures. The five specific objectives of this study are: (1) validate FETAX using direct-acting compounds of known mammalian developmental toxicity, (2) develop and validate an in vitro metabolic activation system using rat liver microsomes, (3) explore and use three carrier solvents which will help solubilize non-polar compounds, (4) develop an Atlas of Abnormalities as a companion manual to the ASTM New Standard Guide for the Conduct of FETAX and, (5) compare the sensitivity of FETAX with a fathead minnow bioassay modified to have the same exposure conditions as FETAX.

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Document Details

Document Type
Technical Report
Publication Date
Feb 28, 1991
Accession Number
ADA236986

Entities

People

  • John A. Bantle

Organizations

  • Oklahoma State University–Stillwater

Tags

DTIC Thesaurus Topics

  • Birds
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Embryos
  • Fish
  • Medical Personnel
  • Organic Chemistry
  • Pharmacology
  • Toxicity

Readers

  • Computational Modeling and Simulation
  • Toxicology/Environmental Toxicology