Molecular Biology of STLV-III and HTLV-IV

Abstract

We have developed a novel technique for rapidly studying microheterogeneity in a population of primate immunodeficiency viruses. Proviruses are rapidly cloned by using an integration - competent cytoplasmic extract from infected cells and integration - sensitive undersize cloning vectors based on bacteriophage lambda. The details of this cloning system are presented. This method yields many clones of integrated proviral DNA, each of which represents an independent clone of provirus contained in the original infected cell population. The utility of this vector to characterize in vitro integration products of a primate immunodeficiency virus (HIV-1) are illustrated as an example.

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Document Details

Document Type
Technical Report
Publication Date
Aug 22, 1990
Accession Number
ADA237035

Entities

People

  • Joseph Sodroski

Organizations

  • Dana–Farber Cancer Institute

Tags

DTIC Thesaurus Topics

  • Bacteriophages
  • Biochemistry
  • Biology
  • Biomedical Research
  • Chemical Compounds
  • Chemistry
  • Classification
  • Coliphages
  • Genetic Vectors
  • Ligation
  • Materials
  • Molecular Biology
  • New England
  • Nucleic Acids
  • Proviruses
  • Security
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Software Engineering