Effects of Abasic Sites on Triple Helix Formation Characterized by Affinity Cleaving
Abstract
The stability of triple helical complexes between oligodeoxyribonucleotides containing one or two abasic 1,2-dideoxy-D-ribose residues bound to single 15-17 base pair sites within short duplex (30mer) or plasmid DNA (4.9 kbp) was examined by affinity cleaving. The triplets are significantly less stable than triplexes having the matched counterparts, T AT, C+GC and G TA. Oligodeoxyribonucleotide-directed triple-helix formation offers a chemical approach for the sequence-specific binding of double-helical DNA that is 10 to the 6th power times more specific than restriction enzymes. Because triple-helix formation by pyrimidine oligodeoxyribonucleotides is limited to mostly purine tracts (T AT, C+GC triplets), it is desirable to find a general solution whereby oligodeoxyribonucleotides (or other analogs) could be used to bind all four base pairs of intact duplex DNA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1991
- Accession Number
- ADA237528
Entities
People
- D. A. Horne
- P. B. Dervan
Organizations
- California Institute of Technology