Surface-Bound Biomembranes Incorporating Receptors: Electrochemical and Structural Characterization.
Abstract
A method is described for forming stable surface-bound structures that incorporate membrane receptors in a lipid bilayer environment. These structures were formed by dialysis of detergent-solubilized biomembranes in the presence of two alkylsilanated substrates: Si/SiO2 and glass beads. Electrochemical analysis of the capacitance was used to determine an apparent thickness and the degree of surface coverage at each stage in the deposition process. Similarly, elemental analysis on glass beads was used to quantitate the carbon load. Glass beads substrates were also examined by FTIR spectroscopy to evaluate the alkylsilanated substrate before and after dialysis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 06, 1991
- Accession Number
- ADA237605
Entities
People
- Elizabeth M. Penniman
- H. G. Smith
- Jianguo Li
- Nancy W. Downer