Neurotransmitter Suppression of the In Vitro Generation of the Cytotoxic T-Lymphocyte Response Against Syngeneic MOPC-315 Plasmacytomas by Spleen Cells from Melphalan-Cured MOPC-315 Tumor Bearers (Catecholamine Inhibition of Tumor Immunity Following Chemotherapy)
Abstract
The effects of the 'stress' -related mediator norepinephrine and related compounds on the generation of anti-tumor cytotoxic activity by spleen cells from melphalin-cured MOPC-315 tumor bearing mice were determined. Cytotoxic activity was generated by cocultrue of spleen lymphocytes from melphalin-cured MOPC-315 tumor bearing BALB/c mice with syngenec MOPC-315 plasmacytoma cells and was assayed by the Cr release assay. At concentrations of 50-100 uM, the catecholamines, norepinephrine, epinephrine, and isoproterenol, and the non-catecholamine, dopamine inhibited the development of cytotoxic activity by 30-96%. Generation of anti-MOPC-315 cytotoxicity was not significantly affected by serotonin or carbachol.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1991
- Accession Number
- ADA237650
Entities
People
- Donald A. Chambers
- Joan M. Cook-mills
- Joseph P. Kennedy Jr.
- Margalit Mokyr
- Robert L. Perlman
Organizations
- University of Illinois at Chicago