Development of Biochemical Cyanide Antidotes
Abstract
Twenty-four different substances were tested as potential cyanide antidotes using cultured rat pheochromocytoma (p-PC12) cells. Prevention of cyanide-induced biochemical changes (increased cell calcium, peroxide formation, cytochrome oxidase inhibition and inhibition of antioxidant defense enzymes) in isolated PC12 cells was used as a measure of in vitro antidotal effectiveness. For comparison, protection against cyanide lethality in mice (LD50) was evaluated for -11 compounds. The linear regression line relating in vitro with in vivo antidotal activity had a correlation coefficient of 0.54. Thus more data are needed to fully evaluate the in vitro system but this rapid screening procedure may prove to be an efficient way to predict whether a substance is a useful cyanide antagonist.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 30, 1990
- Accession Number
- ADA237879
Entities
People
- Gary E. Isom
- Joseph L. Borowitz
Organizations
- Purdue Research Foundation