Structural Role of Amino Acids 99-110 in Recombinant Human Erythropoietin

Abstract

Erythropoietin is the prime regulator of red blood cell production. Mutagenesis of wild type human erythropoietin cDNA was used to produce a series of mutant proteins with sequential deletion of three adjacent amino acids and insertion of the sequence Glu-Phe across the two domains. Transient expression in COS-7 cells revealed 2.0 kb transcripts encoded by all of the cDNas.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1990
Accession Number
ADA238677

Entities

People

  • Arthur J. Sytkowski
  • Taeowan Chung
  • Yijuang Chern

Organizations

  • Beth Israel Deaconess Medical Center

Tags

DTIC Thesaurus Topics

  • Acids
  • Albumins
  • Amino Acids
  • Bioassay
  • Biochemistry
  • Biology
  • Blood
  • Blood Cells
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Molecular Biology
  • New York
  • Nucleic Acids
  • Proteins
  • United States

Readers

  • Immunology
  • Molecular Genetics