A Modular Approach to Protein Design.

Abstract

We have taken a modular approach to study peptide interactions with proteins as a model for protein-protein interactions. Two basic approaches were applied to this problem, and peptides were designed to bind either a specific sequence of double stranded DNA or the PP56lck tyrosine protein kinase. This protein kinase likely is the cytoplasmic effector of CD4, a transmembrane receptor whose aviation in vivo is associated with an increase in cytoplasmic free calcium. Peptides have been synthesized which bind to each of these macromolecules with KDs near 2mM. In addition to binding pp56lck tightly, one peptide has been found to stimulate the activity of this enzyme up to 20-fold. We have shown that ionic interactions are important for this activation, and that these peptides alter the substrate specificity of this enzyme. Due to our greater success with the protein kinase project, we have recently been concentrating our efforts on this project.

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Document Details

Document Type
Technical Report
Publication Date
Jul 18, 1991
Accession Number
ADA239041

Entities

People

  • H. N. Bramson

Organizations

  • University of Rochester

Tags

DTIC Thesaurus Topics

  • Air Force
  • Amino Acids
  • Anatomy
  • Biochemistry
  • Biological Sciences
  • Biology
  • Buildings And Structures
  • Chemistry
  • Enzymes
  • Kinases
  • Military Research
  • Molecular Biology
  • Peptides
  • Protein-Protein Interactions
  • Security
  • Small Molecules
  • Tyrosine

Fields of Study

  • Biology
  • Computer science

Readers

  • Molecular Genetics
  • Molecular and Cellular Biochemistry