The Effects of the ND:YAG Laser on In vitro Fibroblast Attachment to Endotoxin Treated Root Surfaces

Abstract

The purpose of this thesis was to evaluate the effects of the Nd:YAG laser on in vitro fibroblast attachment to endotoxin treated root surfaces and to describe any root surface alterations resulting from the use of the laser. Thirty 4 mm x 4 mm cementum segments were obtained from unerupted third molars. Treatment groups were assigned as follows: (1) non-lased, non-diseased; (2) nonlased, diseased; and (3) lased, diseased. Each group contained ten root segments. The roots were considered diseased after soaking them in endotoxin (E. coli 055:b5 lipopolysaccharide (556 EU/ml)) for 72 hours. The lased, diseased root segments were treated with a neodymium: yttrium, aluminum, garnet (Nd:YAG) laser using a 320 micron contact optic fiber handpiece with an energy setting of 80 mJ at 10 pulses per second for one minute. The fiber was held perpendicular to the root surface. An attempt was made to cover the entire root surface equally during the one minute of exposure.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1991
Accession Number
ADA239258

Entities

People

  • David J. Trylovich

Organizations

  • Air Force Institute of Technology

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Carbon Dioxide Lasers
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Dentistry
  • Laser Applications
  • Laser Beams
  • Lasers
  • Medical Personnel
  • Mouth Diseases
  • Surgery
  • Teeth
  • Tooth Diseases
  • Yag Lasers

Readers

  • Mathematics or Statistics
  • Molecular and Cellular Biology
  • Optical Physics and Photonics.

Technology Areas

  • Directed Energy