Comparative Mutagenicity of Urinary Metabolites of Nitropolycylic Aromatic Hydrocarbons 1-Nitropyrene, 2-Nitrofluoranthene, and 3- Nitrofluoranthene
Abstract
This study compared the mutagenicity of urinary metabolites of prevalent air pollutants nitropyrene, nitrofluoranthene, and nitrofluoranthene. Rats were injected intraperitoneally with specific doses and their urine was analyzed for mutagenicity by an Ames plate incorporation assay using Salmonella Typhimurium. Resulting data yielded revertant excretion rate plots used for comparative analysis. To achieve maximum mutagenicity, 1-NP's urinary metabolites required exogenous activation by S9, 2-NFA's did not vary significantly with S9 activation, while 3-NFA's was decreased by S9 metabolism. 2-NFA's mutagenic metabolites' revertant excretion rate was a quarter of the rates of 3-NFA's and 1-NP's mutagenic metabolites. The strongest mutagenicity of urinary metabolites during the 48 hrs after injection was from 1-NP which was double the amounts from 3-NFA and 2-NFA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1991
- Accession Number
- ADA239486
Entities
People
- Michael N. Boucher
Organizations
- Air Force Institute of Technology