Electron Microscopy to Correlate Cell Structures and Biochemical Activity
Abstract
Electron microscopy demonstrated that desferrioxamine (DFO) appears to act specifically during the late trophzoite and early schizont stages of P. falciparum by preventing nuclear division. This finding is consistent with inhibition of the iron-dependent enzyme ribonucleotide reductase induced by DFO. Inhibition of this enzyme appears to contribute to the antimalarial effect of iron chelate. Immuno-electron microscopy showed liposome-encapsulated malarial antigen that is phagocytosed by macrophages can enter an intracellular compartment in which some of the antigenic epitopes are not degraded by lysosomal enzymes. This indicates the possible existence of a pathway in which liposomal contents can bypass lysosomal degradation. PRBC sequestration and cytoadherence of knobs on PRBC to endothelial cells in cerebral vessels were shown in rhesus monkeys infected with P. coatneyi. This indicates that rhesus monkeys infected with P. coatneyi can be used as a primate model for human cerebral malaria.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 15, 1991
- Accession Number
- ADA239640
Entities
People
- Masamichi Aikawa
Organizations
- Case Western Reserve University