Structure and Expression of Genes for Flavivirus Immunogens.

Abstract

Progress during the reporting period included: (1) Immunization of mice with dengue serotype 1 (DEN-1) structural proteins and a modified form of NS1 expressed in insect cells using a recombinant baculovirus. (2) Immunization of mice with an antigenic subfragment of the DEN-1 E protein expressed in E. coli. (3) Demonstration that the E protein fragment produced in E. coli is capable of eliciting virus neutralizing antibodies when injected with alumina as the adjuvent. (4) Generation of large clones of DEN-1 cDNA using the polymerase chain reaction to amplify reverse transcripts of viral RNA. (5) Determination of experimental conditions for the efficient transfection of BHK cells in culture with purified DEN-1 genomic RNA. (6) Development of a sensitive RNA protection assay to determine the ratio of plus-and minus-strand viral RNA in DEN-1- infected cells.

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Document Details

Document Type
Technical Report
Publication Date
Feb 19, 1991
Accession Number
ADA239830

Entities

People

  • Maurille J. Fournier
  • Thomas L. Mason

Organizations

  • University of Massachusetts Amherst

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Baculoviridae
  • Biochemistry
  • Chain Reactions
  • Chemical Reactions
  • Chemistry
  • Classification
  • Clones
  • Contracts
  • Encephalitis
  • Genetic Engineering
  • Identification
  • Medical Personnel
  • Polymerase Chain Reaction
  • Recombinant Dna
  • Vaccines
  • Virology
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Virology (or Medical Virology).