Toxin Production and Immunoassay Development. 1. Palytoxin
Abstract
One hundred fifty mgs of purified lyngbyatoxin A were collected, isolated and delivered to USAMRIID. The specificity of the palytoxin CIEIA was investigated. Native palytoxin was the most reactive, N-acylated palytoxin and inactivated palytoxin were found to retain 10 to 100% serological reactivity. The marine toxins okadiac acid, tetrodotoxin, and lyngbyatoxin A showed no detectable reactivity in the immunoassay. Monoclonal antipalytoxin antibody 73D3 was found to be effective in neutralizing both in vitro and in vivo cytotoxicity of palytoxin. Synthesis of new palytoxin haptens with linkers at sites other than the terminal amino group were begun. Attempts to synthesize a palytoxin hapten linked at the hydroxal end of the molecule or in developing the oxidation/reduction sequence needed for the tritiation of palytoxin have been unsuccessful. possible corrective measures include the production of an anhydrous sample to minimize water interference.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 18, 1991
- Accession Number
- ADA239837
Entities
People
- T. J. Raybould