Dermal Uptake of Dilute Aqueous Trichloroethylene by Hairless Guinea Pigs
Abstract
Percutaneous absorption was measured in sodium pentobarbital sedated female hairless guinea pigs dermally exposed for 70 min to (14) C labeled trichloroethylene (TCE) present at either low (20- to 110-ppb) or high (100,000- pbb) concentrations in water at 32 deg. A glass dermal exposure chamber was used with an aluminum-coated latex diaphragm to obtain a continuous and completely aqueous exposure of approximately 75% of the surface area of each sedated animal. Dermal permeability constants for TCE (in liters of water cleared of TCE per cu. cm hr of exposure) were estimated for low- and high-concentration animal-exposure experiments based on the measured rate of radiolabel loss from chamber water after controlling for the amount of such loss observed in control exposures in which a glass beaker was used in place of a live animal. Uptake was verified by measurement of radiolabel in urine and feces for up to 4 weeks postexposure. The average permeability constant for extremely dilute aqueous TCE obtained from 5 animal experiments using this dermal exposure system was 0.00014 liters cu. cm hr; this value was not significantly different from the corresponding mean value obtained from 5 animal experiments conducted using 100, 000 ppb aqueous TCE. The observed clearance of (14) C-TCE from chamber water was approximately linear over the dermal exposure period.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1990
- Accession Number
- ADA240098
Entities
People
- B. W. Colston Jr.
- K. T. Bogen
- L. K. Machicao
Organizations
- Lawrence Livermore National Laboratory