Genetic Toxicity Assessment of Chloropentafluorobenzene

Abstract

Chloropentalfluorobenzene (CPFB), a substituted halogenated benzene, was evaluated in in vitro bioassays to assess its potential genotoxic activity. The assays conducted were the Ames Salmonella/microsomal mutagenicity assay, the Chinese hamster ovary (CHO) gene mutation assay, the CHO/sister chromatid exchange (SCE) and chromosome aberration assay, the BALB/c-3T3 cell transformation assay, and an in vivo/in vitro unscheduled DNA synthesis assay. CPFB did not demonstrate genotoxic potential in any assays with the exception of the SCE assay in the presence of metabolizing enzymes. The skin sensitization and acute inhalation toxicity potential of CPFB has been found to be unremarkable. However, it did produce mild skin and conjunctival irritation in treated rabbits. Repeated inhalation resulted in a reduced rate in growth of rats but not of mice. Histologically the tissue exhibited mild hepatocytomegaly with increased granular eosinophilic cytoplasm. Bone marrow samples from the exposed mice were evaluated for evidence of CPFB induced genetic changes; all findings were negative.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1990
Accession Number
ADA240099

Entities

People

  • B. C. Myhr
  • D. C. Valentine
  • R. R. Young
  • R. S. Kutzman
  • T. E. Lawlor

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Biomedical Research
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Chemical Warfare Agents
  • Chemistry
  • Chromosome Structures
  • Chromosomes
  • Culture Media
  • Culture Techniques
  • Genetics
  • Mutagenicity Tests
  • Mutations
  • Toxicity

Fields of Study

  • Biology

Readers

  • Toxicology/Environmental Toxicology

Technology Areas

  • Biotechnology