Verification, Dosimetry and Biomonitoring of Mustard Gas Exposure via Immunochemical Detection of Mustard Gas Adducts to DNA and Proteins.
Abstract
The recent use of mustard gas in the Gulf War has stressed the need of reliable methods for retrospective detection of poisoning with chemical warfare agents. We are developing methods to detect adducts of mustard gas to DNA and proteins, which may have life span of many days after exposure. In order to achieve high sensitivity and specificity, immunochemical detection techniques are developed. As markers to detect adducts, we synthesized N7-(2'- hydroxyethylthioethyl)-guanine, the di-adduct di-(2-guanin-7'-yl-ethyl) sulfide, and N3-(2'-hydroxyethylthioethyl)-adenine, 06-(2'-hydroxyethylthioethyl)-guanine and the corresponding 2'-deoxyguanosine derivative. N7-(2'- hydroxyethylthioethyl)-guanine. Mustard Gas Hemoglobin-adducts, Mustard Gas DNA- antiserum, Sulfur Mustard, DNA, Protein. Alkylation, Retrospective detection, Dosimetry, Biological dosimetry of Mustard Gas exposure, Biomonitoring, Verification.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1990
- Accession Number
- ADA240471
Entities
People
- Hendrik P. Benschop
Organizations
- Prins Maurits Laboratorium TNO