Genotoxicity Assessment of Chlorotrifluoroethylene Tetramer Acid using a Battery of In Vitro and In Vivo/In Vitro Assays
Abstract
Chlorotrifluoroethylene (CTFE) tetramer acid, the perhalogenated fatty acid metabolite of the eight-carbon oligomer of polychlorotrifluoroethylene (pCTFE), was evaluated in in vitro bioassays to assess its potential genotoxic activity. The assays conducted were the Ames Salmonella/microsomal mutagenicity assay, the Chinese hamster ovary (CHO) gene mutation assay, the CHO/sister chromatid exchange (SCE) and chromosome aberrations assays, the BALB/c-3T3 cell transformation assay and in vivo/in vitro unscheduled DNA (UDS) and S-phase synthesis assays. CTFE tetramer acid did not demonstrate genotoxic potential in any of the in vitro assays and was also negative in the UDS assay. However, a small, but significant, dose-related increase in S-phase synthesis occurred 48 hours following oral administration of doses of CTFE tetramer acid ranging from 11 to 44 mg/kg. This increase in S- phase synthesis indicated a rapid response to hepatotoxicity caused by CTFE tetramer acid but the results of the test battery would predict no genetic risk from exposure.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1990
- Accession Number
- ADA240492
Entities
People
- C. Myhr
- C. S. Godin
- H. Murli
- R. R. Young
- T. E. Lawlor