Human Immune Response to Dengue Infections.

Abstract

We have mapped the epitopes recognized by the serotype-cross-reactive clone JK34 is located within amino acids (a.a.) 251-165 of NS3. The epitopes recognized by the serotype-crossactive clone JK32 and the flavivirus crossreactive clone JK43 are located within a.a. 1-452 and a.a.1-183 of NS3, respectively. We also established 16 dengue virus-specific CD8+ CD4- T cell clones. The clone 2.8 recognized dengue virus types 2 and 4, and the clone 2.9 recognize four serotypes of dengue viruses. The clones 2.8 and 2.9 recognize NS3 protein, and the epitope recognized by the clone 2.8 is located within a.a. 453- 618. These results indicate that NS3 contains multiple dominant T cell epitopes recognized by CD4+ and CD8+ human CTL. To analyse the mechanisms of antibody- dependent enhancement, we prepared bispecific antibodies.

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Document Details

Document Type
Technical Report
Publication Date
Jun 30, 1991
Accession Number
ADA240717

Entities

People

  • Francis A. Ennis

Organizations

  • University of Massachusetts

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Antigens
  • Blood
  • Cells
  • Dead Reckoning
  • Dengue
  • Diseases And Disorders
  • Infection
  • Interferon
  • Lymphocytes
  • Macrophages
  • Molecules
  • Monocytes
  • Myeloid Cells
  • Proteins
  • T Lymphocytes

Fields of Study

  • Biology

Readers

  • Immunology
  • Virology (or Medical Virology).