Bimodal Effect of Phorbol Ester on B Cell Activation.

Abstract

The role of protein kinase C PKC in B cell activation is controversial. These studies were undertaken to determine whether protein kinase C has a stimulatory of inhibitory role in b cell activation. We found that treatment of B cells for a short period of time (30 min) with the PKC activator phorbol 12,13-dibutyrate (PDBU) primed the cells for enhanced proliferative responses to anti-immunoglobulin (anti-Ig) antibody whereas treatment for a longer period of time (3 hr or more) resulted in suppression of proliferation. the enhanced proliferative response to treatment of B cells with PDBU for short periods of time was associated with inhibition of anti-Ig-stimulated increases in phosphatidyl 4,5-bisphosphate (PIP2)hydrolysis and inhibition of increases in (Ca2+), indicating that activation of PKC per se might be sufficient for enhancing b cell activation.

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Document Details

Document Type
Technical Report
Publication Date
Mar 05, 1991
Accession Number
ADA240837

Entities

People

  • Anil K. Balapure
  • Carl H. June
  • James J. Mond
  • Nili Feuerstein
  • Robert I. Glazer

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Antibodies
  • B Lymphocytes
  • Biomedical Research
  • Blood
  • Body Fluids
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Esters
  • Immunoglobulins
  • Inhibition
  • Lymphatic System
  • Organic Chemistry
  • Proteins
  • Sugar Alcohols
  • Tissues

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Immunology
  • Mathematics or Statistics