Mechanisms of Action of Clostridial Neurotoxins on Dissociated Mouse Spinal Cord Neurons in Cell Culture
Abstract
The mechanism of action of tetanus toxin was investigated using the in vitro system of dissociated mouse spinal cord neurons in culture. Monoclonal antibodies directed against various epitopes on the toxin molecule were investigated with regard to their ability to neutralize the convulsant effects of the toxin. Of the antibodies tested, neutralizing antibodies were found that were directed against the binding subunit (fragment C) of the toxin as well as the heavy chain portion of the nonbinding subunit (fragment B). These findings suggest that the active domain of the toxin molecule resides on the B subunit. Other experiments utilizing agents to potential antagonize tetanus toxin revealed that chloramine was toxic to the neurons, ammonium chloride produced convulsant activity itself, Concancavalin A appeared to block synaptic transmission itself and cGMP failed to reverse the effects of the toxin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 15, 1988
- Accession Number
- ADA241319
Entities
People
- Gregory K. Bergey
Organizations
- University of Maryland School of Medicine