Cloning and Expression of Genes for Dengue Virus Type-2 Encoded-Antigens for Rapid Diagnosis and Vaccine Development

Abstract

The complete sequence of dengue virus type 2 (New Guinea strain C) has been determined. The genome is 10,723 nucleotides long. The organization of the genome is similar to that of yellow fever virus. The neutralization epitope of a monoclonal antibody 3H5 has been mapped to a region of 12 amino acids QLKLNWFKKGSS located in E protein of DEN-2 virus. The antibodies raised against this peptide can neutralize the infectivity of the virus, although these results need to be confirmed. The peptide antisera can mimic 3H5 in other tests. The peptide also reacts with 3H5 with specificity. We have obtained experimental evidence which demonstrate more directly that the 3'-terminus of DEN-2 RNA has the potential to form secondary structure in solution. This finding has important implications for flavivirus replication.

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Document Details

Document Type
Technical Report
Publication Date
Aug 26, 1991
Accession Number
ADA241648

Entities

People

  • Radha K. Padmanabhan

Organizations

  • University of Kansas Medical Center

Tags

DTIC Thesaurus Topics

  • Antigens
  • Arbovirus Infections
  • Arthropod-Borne Encephalitis
  • Chemistry
  • Dengue
  • Encephalitis
  • Flavivirus Infections
  • Gene Expression
  • Medical Personnel
  • Molecular Biology
  • New Guinea
  • Ribonucleic Acids
  • Rna Viruses
  • Sequence Analysis
  • Vaccines
  • Viruses
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology