Recognition Using Biospecific Interaction Analysis
Abstract
Biospecific interaction analysis using BIAcore was employed to assess the possibility of recognizing specific antigen within a cellular milieu containing complex saccharides and lipopolysaccharides. Monoclonal antibodies to adhesion proteins of 'Entamoeba histolytica' were used as capture ligand. These monoclonal antibodies, directed against specific epitopes on the adherence lectin, can distinguish between pathogenic and nonpathogenic strains. Amebic preparations were solubilized with detergent in buffer, and this preparation was used as the challenge mixture. The results suggest interference with the binding of antigen to immobilized antibody caused by micelle formation, size difference, or an insufficient amount of adherence protein.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1991
- Accession Number
- ADA241879
Entities
People
- Russ Granzow
- Sheila J. Wood
- William Petri Jr.
Organizations
- Edgewood Chemical Biological Center