Kullback-Leibler Information for Ordering Genes Using Sperm Typing and Radiation Hybrid Mapping
Abstract
Two technologies applicable to gene mapping are those of sperm typing and radiation hybrid mapping. Sperm typing makes use of the polymerase chain reaction, a biochemical technique which allows enormous amplification (production of multiple copies) of small, selected DNA fragments from a single chromosome. A sample of sperm from a single donor is analyzed to see which alleles (distinct forms of the various genes) are present in the individual sperms. The frequencies with which the various possibilities occur can be used to supply estimates of the ordering and of the recombination probabilities among the genes for which that donor is heterozygous (having different alleles of the same gene.)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1991
- Accession Number
- ADA241982
Entities
People
- Herman Chernoff
Organizations
- Harvard University