Comparative Infectivity Determination of Dengue Virus Vaccine Candidates in Rhesus Monkeys, Mosquitoes, and Cell Culture
Abstract
On February 12, 1991, sixteen rhesus monkeys were sent from the Caribbean Primate Center (Sabana Seca) to the Animal Resources Facilities at the Medical Sciences Campus. The monkeys were bled on February 12, and on February 26, and the sera were stored at -20 C. The Plaque Reduction Neutralization test (PRNT) and the hemagglutination inhibition (HI) test were performed using these serum samples (Pre vaccination bleeds). The PRNT was performed using LLC-MK2 cells (sent by Dr. Goro Kuno CDC, San Juan Laboratories) grown in multiwell plates as described before. Vaccines and the other materials were received on 4/ 4/91. On April 22, 1991 groups of four monkeys were innoculated s.c. with 0.5 ml of each candidate vaccine. The titration of the four vaccine candidates were made on the same day of inoculation using LLC-MK2 cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 28, 1991
- Accession Number
- ADA242097
Entities
People
- Edmundo Kraiselburd
Organizations
- Purdue University