Changes in Rabbit Sperm Motion Characteristics during Incubation and Its Use in the Assessment of Chemical Cytotoxicity

Abstract

The CellSoft Motion Analysis System was used to study the changes in motion characteristics of rabbit sperm during incubation in a medium in which capacitation occurs. Sperm velocity reached a maximum after 1 hr of incubation and then declined linearly. The mean velocity at 5 hr was significantly higher than the mean at o hr. A steady increase in average amplitude of lateral head (alh) was observed, the values at 0 and 5 hr being significantly different. The motility characteristics of individual sperm cells also changed during incubation; however, this change was not reflected in the motion parameters. Positive correlations were observed only between curvilinear and straight line velocities and between maximum and average alh. A new toxicological endpoint, motility concentration index50, MCI50, was defined.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1991
Accession Number
ADA242467

Entities

People

  • Barry A. Bodt
  • Elizabeth A. Laurie
  • R. J. Young
  • W. C. Starke

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amplitude
  • Animals
  • Biological Sciences
  • Cell Movement
  • Cells
  • Dermatologic Agents
  • Engineering
  • Frequency
  • Incubation
  • Indicator Dyes
  • Laboratory Animals
  • Lethal Dosage
  • Long Wavelengths
  • Physiology
  • Rotation
  • Technical Information Centers

Readers

  • Aerodynamics.
  • Mathematics or Statistics
  • Toxicology/Environmental Toxicology