Development of Hematopoietic Growth Factors for Use in Military Personnel
Abstract
We have continued our work on the new series of erythropoietin mutants. Interestingly, in sequencing the first five mutants that we derived, we discovered that another mutation had occurred spontaneously. In the short term, this has resulted in some delay in our studies, since we now feel it necessary to sequence not only the mutated domain of each cDNA, but rather the entire cDNA to verify the absence of other spontaneous mutations. However, in the long term, it is to our benefit to realize that the state-of-the-art methodology that we have employed in preparing these mutants is subject to some technical artifacts. Discovering this at this early stage of our studies will save us many problems in the long run. We expect that within the next three to six months, we will have finished all of our sequencing and will begin to express some of these mutants and initiate their biological characterization.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 12, 1991
- Accession Number
- ADA242475
Entities
People
- Arthur J. Sytkowski
Organizations
- Beth Israel Deaconess Medical Center