Detection and Clinicopathologic Correlation of Human Immunodeficiency Virus (HIV-1) Nucleic Acids and Antigens in Reticuloendothelial and Central Nervous System Tissues, by Immunohistochemistry, in situ Hybridization, and Polymerase Chain Reaction
Abstract
The pathologic findings os postmortem lymph nodes (LN) from supraclavicular, axillary, mediastinal, mesenteric, and inguinal sites were compared in 5 Western blot confirmed HIV infected asymptomatic intravenous drug addicts (IVDA) and 8 HIV seronegative control IVDA. The predominant LN pattern was classified as follicular hyperplasia (FH), sinus histiocytosis (SH), and lymphoid depletion (LD). the percent distribution of these histologic changes in HIV positive (and HIV negative) individuals was 64% (47%) FH, 20% (31%) LD, and 15% (22%) SH. Plasmacytosis was prominent in 15 of 25 (60%) LN from HIV seropositive individuals, but absent in controls. Frozen and paraffin immunohistochemistry of HIV+ LN with FH demonstrated p24 antigen within germinal centers in a distribution similar to CD35+ endritic reticulum cells, but not in controls. HIV-1 RNA could not be detected by in situ hybridization in LN which were HIV DNA positive by polymerase chain reaction, probably because of postmortem RNA degradation, but was demonstrated in all HIV-positive surgically acquired control tissues. LN changes in early HIV infection are nonspecific.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 02, 1991
- Accession Number
- ADA242818
Entities
People
- Allen P. Burke
Organizations
- Armed Forces Institute of Pathology