Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes

Abstract

There are three main objectives of the research. First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig- genes into human hematopoietic progenitors and then grow and activate those cells in an animal model.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1991
Accession Number
ADA243424

Entities

People

  • James J. Kenny

Tags

DTIC Thesaurus Topics

  • Biomedical And Dental Materials
  • Blood
  • Blood Cells
  • Bone Marrow
  • Bone Marrow Cells
  • Cardiovascular System
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Chromosomes
  • Genetics
  • Growth Factors
  • Lymphatic System
  • Lymphocytes
  • Stem Cells
  • Thymocytes

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech