Enzymatic Determination of Phosphatidylglycerol in the 10,000 x g Pellet of Amniotic Fluid
Abstract
When present in amniotic fluid, phosphatidylglycerol almost indicated maturity of the fetal lungs. Enzymatic analysis can improve detection sensitivity by measuring the concentration of phosphatidylglycerol in amniotic fluid. By centrifuging (10,000 x g) amniotic fluid to obtain a lamellar body pellet, the phosphatidylglycerol content of the pellet, the phosphatidlglycerol content of the pellet can be determined and compared to established indicators fetal lung maturity. To enhance the detection of phosphatidylglycerol, lamellar body pellets are reconstituted with less buffer than removed fluid to improve the sensitivity of enzymatic testing. During the study, 128 amniotic fluids were evaluated by comparing the lecithin/sphingomylein ratio, fluorescence polarization, extracted enzymatic phosphatidyglycerol, and centrifuged (10,000 x g) enzymatic phosphatidylglycerol.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1991
- Accession Number
- ADA243601
Entities
People
- George W. Jones
Organizations
- Air Force Institute of Technology