Glutamate/NMDA Receptor Ion-Channel Purification, Molecular Studies and Reconstitution into Stable Matrices

Abstract

This is the final report of a project that had as its primary focus the isolation of the NMDA receptor protein complex and the reconstitution of these proteins for the development of biosensors. The progress made in all phases of this work has met the goals selected for the research. (1) Two proteins of the receptor were fully purified and characterized. (2) The intact complex was purified and biochemically characterized. (3) One of the protein CDNA's was cloned and sequenced. (4) The complex was reconstituted into liposomes and planar lipid bilayers. (5) A prototype sensor based on this receptor complex was developed. (6) Cell model systems for the study of the receptor were developed.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 29, 1991
Accession Number
ADA244727

Entities

People

  • Elias K. Michaelis

Organizations

  • University of Kansas

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Biochemistry
  • Biosensors
  • Carrier Proteins
  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Detectors
  • Glutamates
  • Lipids
  • Membrane Lipids
  • Models
  • Proteins
  • Prototypes
  • Scientists
  • Students
  • Synapses

Readers

  • Molecular and Cellular Biochemistry
  • Software Engineering

Technology Areas

  • Biotechnology