Analysis of Saxitoxin from Urine Using Dynamic FAB/MS

Abstract

An improved method of saxitoxin analysis in urine using continuous Flow Fast Atom Bombardment Mass Spectrometry (CF/FAB/MS) was developed. Parameters studied were matrix composition, matrix flow rate, temperature of probe tip, probe tip design and sample extraction. Optimal detection was obtained using the following matrix composition: 5% glycerol, 0.5% acetic acid, 0.025% sodium dodecylsulfate, 0.1% polyethylene glycol (PEG) 400 and 0.5% PEG 300; probe tip temperature: (-55 C); flow rate: 5 or 8 microliter per min; probe tip: Olson-Hogge design. The STX standard was detected at 200 picograms with signal-to-noise ratio of 11. The percent recovery of saxitoxin from human urine after cleanup on a weak cation exchange (CBA Bond-Elut Analytichem Inter. Varian, Anaheim, CA) column was 75%.

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Document Details

Document Type
Technical Report
Publication Date
Oct 21, 1991
Accession Number
ADA244960

Entities

People

  • Chester J. Mirocha
  • Hamed K. Abbas
  • Urooj Mirza
  • Weiping Xie
  • Young B. Kim

Organizations

  • University of Minnesota

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetic Acid
  • Alcohols
  • Capillary Electrophoresis
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Flow Rate
  • Glycerols
  • Liquid Chromatography
  • Mass Spectra
  • Mass Spectrometers
  • Mass Spectrometry
  • Molecules
  • Polyethylene Glycols
  • Spectrometers
  • Spectrometry
  • Sugar Alcohols

Readers

  • Aerodynamics.
  • Analytical Chemistry