Effects of Pressure on Membrane-Associated Receptors and Effector Element
Abstract
To investigate the effects of moderate hydrostatic pressures on signal transduction we have used the A (1) adenosine receptor inhibitory G protein (Gi) - adenylyl cyclase system in two species of sorpaenid fish which have served as a model for the study of pressure adaptation. These species, Sebastolobus alascanus and S. Altivelis, have similar body temperatures, but dwell at different depths, and thus experience different hydrostatic pressures. The experiments were designed to identify and define at the molecular level the effects of pressure on the components of the signal transduction system in isolation and on the entire functional complex. We have completed work characterizing the effects of pressure on the coupling efficiency of the A (1) adenosine receptor in brain membrane preparations. For the teleost brain membrane preparations, incubation at 5 C and 476 atm does not result in loss of adenylyl cyclase activity or coupling to the A (1) adenosine receptor on subsequent assays at atmospheric pressure. In contrast, rat brain membrane preparations lost 59% of their activity under these conditions.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 31, 1991
- Accession Number
- ADA245220
Entities
People
- Thomas F. Murray
Organizations
- Oregon State University