Role of Protein Methylation in Halobacterium halobium Phototaxis.
Abstract
The objective of this grant was to investigate the role of methyl-accepting proteins in the phototaxis signaling system of H. halobium membranes. A carboxylmethylated protein in the membrane, MPP-I (Methyl-accepting phototaxis protein I) appears to relay the signal from photoactivated sensory rhodopsin I (SR-I, a visual pigment-like photosensor). Our primary objective was to elucidate the relationship between SR-I and MPP-I. Accomplishments in this period have been in 3 areas: (1) Signaling conformations of SR-I have been studied and processes important to receptor activation identified; (2) Tryptic peptide sequence has been obtained from MPP-I and the gene encoding MPP-I cloned; and (3) in collaboration with the laboratory of H. G. Khorana, we have designed, synthesized and expressed an SR-I gene optimized for site-directed mutagenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 21, 1992
- Accession Number
- ADA245397
Entities
People
- John L. Spudich
Organizations
- Albert Einstein College of Medicine