Examination of Chemical Adsorption and Marine Biofouling on Metal Surfaces Using Raman Scattering Techniques and Electrochemical Impedance Spectroscopy
Abstract
A suite of spectroscopic, optical, and electrochemical techniques to examine initial stages of biofilm formation were developed and evaluated. Using a combination of ellipsometry, FT-IR reflectance-absorbance spectroscopy, and electrochemical impedance spectroscopy, we were able to gain quantitative, compositional, and structural information about the substratum-adsorbate interface for the model protein, Ribulose 1,5-Bisphosphate Carboxylase- Oxygenase. Adsorption isotherms for RuBisCO on titanium, copper, iron, glass, polycarbonate, and teflon were obtained. Spectroscopic evidence suggests that molecular structure and electrical impedance vary with protein surface coverage. Furthermore, bioavailability of sorbed protein and degradation rates appear to vary with coverage resulting from altered structural conformation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 14, 1991
- Accession Number
- ADA245511
Entities
People
- Gordon T. Taylor