Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes

Abstract

First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to be activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig-genes into human hematopoietic progenitors and then grow and activate those cells in an animal model.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1992
Accession Number
ADA245750

Entities

People

  • James J. Kenny

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Blood
  • Bone Marrow
  • Bone Marrow Cells
  • Bones
  • Cell Line
  • Cells
  • Immune System
  • Immunogenetic Phenomena
  • Immunoglobulins
  • Lymphatic System
  • Stem Cells

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech